antibody

Sentrin 1 (SUMO-1, SMT3C)

S0750-01K

100 ul

polyclonal

domestic rabbit

nonconjugated

Sentrin 1 (SUMO-1, SMT3C)

rabbit

Pab

Antibodies

100 ul

IgG

Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (sumoylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process and is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin regulates predominantly degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and I B- to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP and regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer's disease (8-11). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:50. Antigen retrieval: citrate method. Antibody diluent: TBST/5% normal goat serum Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide derived from a sequence within human SUMO-1 that does not correspond to SUMO-2/3.

Detects recombinant SUMO-1 and and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD). Species Crossreactivity: Human, mouse, monkey and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide derived from a sequence within human SUMO-1 that does not correspond to SUMO-2/3. Specificity: Detects recombinant SUMO-1 and and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD). Species Crossreactivity: Human, mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IHC IP WB

-20°C

(1) Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321–328. (2) Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257–268. (3) Matunis, M.J. et al. (1996) J. Cell Biol. 135, 1457–1470. (4) Duprez, E. et al. (1999) J. Cell Sci. 112, 381–393. (5) Gostissa, M. et al. (1999) EMBO J. 18, 6462–6474. (6) Rodriguez, M.S. et al. (1999) EMBO J. 18, 6455–6461. (7) Desterro, J.M. et al. (1998) Mol. Cell 2, 233–239. (8) Tatham, M.H. et al. (2001) J. Biol. Chem. 276, 35368–35374. (9) Azuma, Y. et al. (2003) J. Cell Biol. 163, 477–487. (10) Li, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 259–264.(11) Saitoh, H. and Hinchey, J. (2000) J. Biol. Chem. 275,6252–6258.

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