antibody

SAPK, JNK (Stress-Activated Protein Kinase, Jun-terminal Kinase)

S0096-04E

200 ul

polyclonal

domestic rabbit

nonconjugated

SAPK, JNK (Stress-Activated Protein Kinase, Jun-terminal Kinase)

rabbit

Pab

Antibodies

200 ul

IgG

The stress-activated protein kinase/Jun terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1–4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4–7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4–7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Bo Hm Hu Mk Mo Rt Ze

Fusion protein corresponding to human JNK2 and GST.

Detects endogenous levels of total SAPK/JNK protein. Species Crossreactivity: Human, bovine, mouse, hamster, monkey, rat and zebrafish.

Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Fusion protein corresponding to human JNK2 and GST. Specificity: Detects endogenous levels of total SAPK/JNK protein. Species Crossreactivity: Human, bovine, mouse, hamster, monkey, rat and zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1–12. 2. Ichijo, H. (1999) Oncogene 18, 6087–6093. 3. Kyriakis, J.M. and Avaruck, J. (2001) Physiol. Rev. 81, 807–869. 4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259–5262. 5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158–6162. 6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481–485. 7. Banerjee, S. et al. (2002) J. Virol. 76, 5937–5948. 8. Brouard, S. et al. (2000) J. Exp. Med. 192, 1015–1026. 9. Chen, F. et al. (2001) J. Biol. Chem. 276, 11414–11419. 10. Dai, Y. et al. (2001) Cancer Res. 61, 5106–5115. 11. Hebert, S.S. et al. (2000) J. Biol. Chem. 275, 32482–32490. 12. Lee, E.G. et al. (2000) Science 289, 2350–2354. 13. Park, Y.C. et al. (2000) Cell 101, 777–787.

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