SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase) | US Biological S0096-04C product information

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product summary

company name :

US Biological

product type :

antibody

product name :

SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

catalog :

S0096-04C

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3A33(G9)

reactivity :

human

product information

Catalog Number :

S0096-04C

Product wo Prefix :

SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

Host :

mouse

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

200 ul

Clone # USB :

3A33(G9)

Isotype :

IgG1

Desc1 :

The stress-activated protein kinase/Junterminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1-4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4-7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4-7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors. Application: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000. Incubate membrane with diluted S0096-04C in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:250 Flow Cytometry: 1:400 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hm Hu Mo Rt Ye

Immunogen :

Synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK.

Specificity :

Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species Crossreactivity: mouse, rat, hamster, S. cerevisiae.

Purity :

Purified by immunoaffinity chromatography from ascites.

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3A33(G9) Host: mouse Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified by immunoaffinity chromatography from ascites. Immunogen: Synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK. Specificity: Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species Crossreactivity: mouse, rat, hamster, S. cerevisiae. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IP WB

Storage Temperature :

-20°C

Reference :

1. Davis, R.J., Biochem. Soc. Symp. 64: 1-12 (1999). 2. Ichijo, H., Oncogene 18: 6087-6093 (1999). 3. Kyriakis, J.M., Avaruck, J., Physiol. Rev. 81: 807-869 (2001). 4. Kyriakis, J.M., J. Biol. Chem. 274: 5259-5262 (1999). 5. Leppa, S., Bohmann, D., Oncogene 18: 6158-6162 (1999). 6. Whitmarsh, A.J., Davis, R.J., Trends Biochem. Sci. 23: 481-485 (1998). 7. Akagi, T., et al., PNAS USA 97: 7290-7295 (2000). 8. Saporito, M.S., et al., J. Neurochem. 75: 1200-1208 (2000). 9. Somasundaram, S., et al., Cancer Res. 62: 3868-3875 (2002). 10. Song, J.S., et al., J. Immunol. 163: 802-810 (1999). 11. Xing, J., et al., Mol. Cell. Biol. 18: 1946-1955 (1998). 12. Zhang, S., Kaplan, M.H., J. Immunol. 165: 1374-1380 (2000).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/46/S0096-04C_1.jpg