SAPK, JNK , phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase) | US Biological S0096-04A product information

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product summary

company name :

US Biological

product type :

antibody

product name :

SAPK, JNK , phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

catalog :

S0096-04A

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

S0096-04A

Product wo Prefix :

SAPK, JNK , phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

200 ul

Isotype :

IgG

Desc1 :

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Bo Hm Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK (KLH). Species sequence homology: Xenopus

Specificity :

Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185 between 46-54kD. Does not recognize unphosphorylated SAPK/JNK. Species Crossreactivity: mouse, monkey, hamster, bovine, rat, Drosophila.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK (KLH). Species sequence homology: Xenopus Specificity: Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185 between 46-54kD. Does not recognize unphosphorylated SAPK/JNK. Species Crossreactivity: mouse, monkey, hamster, bovine, rat, Drosophila. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1–12. 2. Ichijo, H. (1999) Oncogene 18, 6087–6093. 3. Kyriakis, J.M. and Avaruck, J. (2001) Physiol. Rev. 81, 807–869. 4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259–5262. 5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158–6162. 6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481–485. 7. Kujime, K. et al. (2000) J. Immunol. 164, 3222–3228. 8. Rochat-Steiner, V. et al. (2000) J. Exp. Med. 192, 1165–1174. 9. Shukla, A. et al. (2001) Cancer Res. 61, 1791–1795. 10. Weiss, L. et al. (2000) J. Exp. Med. 191, 139–146.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/28/S0096-04A_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S0096-04A Antibody.