antibody

Salmonella

S0060-12A

250 ul

polyclonal

domestic rabbit

nonconjugated

Salmonella

rabbit

Pab

Antibodies

250 ul

IgG

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:10-1:500 Immunohistochemistry (Frozen/Paraffin): 1:10-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Mixture of S. enteriditis, S. typhimurium and S. heidelburg. Cellular Localization: Whole cell

Recognizes Salmonella. Polyvalent for Salmonella O and H antigens. Does react with related Enterobacteriaceae.

Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

~4mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~4mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: Mixture of S. enteriditis, S. typhimurium and S. heidelburg. Cellular Localization: Whole cell Specificity: Recognizes Salmonella. Polyvalent for Salmonella O and H antigens. Does react with related Enterobacteriaceae. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IC IF IHC

-20°C

General References: 1. Giannella RA (1996). Salmonella. In: Baron's Medical Microbiology (Barron S et al., eds.) (4th ed.). Univ of Texas Medical Branch. (via NCBI Bookshelf) ISBN 0-9631172-1-1. 2. Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0-8385-8529-9. 3. Parkhill J et al. (2001). "Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18". Nature 413 (6858): 848–52. doi:10.1038/35101607. PMID 11677608 doi:10.1038/35101607. 4. McClelland M et al. (2001). "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2". Nature 413 (6858): 852–6. doi:10.1038/35101614. PMID 11677609 doi:10.1038/35101614. 5. Adkins JN et al. (2006). "Analysis of the Salmonella typhimurium Proteome through Environmental Response toward Infectious Conditions". Molecular and Cellular Proteomics 5: 1450–1461. doi:10.1074/mcp.M600139-MCP200. PMID 16684765. 6. Huang H, Li J, Yang XL, et al. (January 2009). "Sequence Analysis of the Plasmid pGY1 Harbored in Salmonella enterica Serovar Paratyphi A". Biochem. Genet. 47: 191. doi:10.1007/s10528-008-9216-0. PMID 19169860. 7. Swanson SJ, Snider C, Braden CR, et al. (2007). "Multidrug-resistant Salmonella enterica serotype Typhimurium associated with pet rodents". New England Journal of Medicine 356 (1): 21–28. doi:10.1056/NEJMoa060465. PMID 17202452. 8. Hensel M (2009). "Secreted Proteins and Virulence in Salmonella enterica". Bacterial Secreted Proteins: Secretory Mechanisms and Role in Pathogenesis. Caister Academic Press. ISBN 978-1-904455-42-4. http://en.wikipedia.org/wiki/Salmonella_typhimurium