RSK1-3 (RSK-1, RSK-2, RSK-3, Ribosomal S6 Kinase, MAPKAP K1a, MAPKAP K1b) | US Biological R9399-16 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

RSK1-3 (RSK-1, RSK-2, RSK-3, Ribosomal S6 Kinase, MAPKAP K1a, MAPKAP K1b)

catalog :

R9399-16

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H61(32D7)

reactivity :

human

product information

Catalog Number :

R9399-16

Product wo Prefix :

RSK1-3 (RSK-1, RSK-2, RSK-3, Ribosomal S6 Kinase, MAPKAP K1a, MAPKAP K1b)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H61(32D7)

Isotype :

IgG

Desc1 :

The 90kD ribosomal S6 kinases (RSK1- 3) are a family of broadly expressed serine/threonine kinases activated by extracellular signal-regulated protein kinases (Erk1 and 2) in response to many growth factors, polypeptide hormones and neurotransmitters (1). p90RSK is activated by MAPK in vitro and in vivo via phosphorylation (4). Several sites, such as Ser380, Thr359, Ser363 and Thr573 are important for its activation (3). RSK3 resembles p90RSK by having two kinase domains connected by a regulator linker region (2). Phosphorylation of p90RSK (and likely RSK2 and RSK3) in the linker region is critical for its activity (3). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Gp Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Asp659 of human p90RSK.

Specificity :

Recognizes endogenous levels of total human RSK1/RSK2/RSK3 proteins. Species Crossreactivity: mouse, rat, monkey and guinea pig

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H61(32D7) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Asp659 of human p90RSK. Specificity: Recognizes endogenous levels of total human RSK1/RSK2/RSK3 proteins. Species Crossreactivity: mouse, rat, monkey and guinea pig Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IP WB

Storage Temperature :

-20°C

Reference :

(1) Frodin, M. and Gammeltoft, S. (1999) Mol. Cell. Endocrinol. 151, 65–77. (2) Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661–29671. (3) Dalby, K. et al. (1998) J. Biol. Chem. 273, 1496–1505. (4) Lazar, D.F. et al. (1995) J. Biol. Chem. 270, 20801–20807.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/7/R9399-16_1.jpg