antibody

RPA, p70 (DNA Replication, Repair and Recombination Factor)

R3400-01Q

100 ul

monoclonal

rat

nonconjugated

7H59(4D9)

RPA, p70 (DNA Replication, Repair and Recombination Factor)

rat

Mab

Antibodies

100 ul

7H59(4D9)

IgG1

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14kD subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70kD subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1,2,3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4,5,6,7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9,10,11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol , and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (12,10). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk

Recombinant MBP-fusion protein corresponding to the C-terminal sequence of human RPA70.

Detects endogenous levels of total human RPA70 protein. Species Crossreactivity: monkey

Serum

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 7H59(4D9) Host: rat Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Serum. Immunogen: Recombinant MBP-fusion protein corresponding to the C-terminal sequence of human RPA70. Specificity: Detects endogenous levels of total human RPA70 protein. Species Crossreactivity: monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IP WB

-20°C

(1) Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222–7231. (2) Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834–1838. (3) Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211–1218. (4) Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523–2527. (5) Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433–439. (6) Kastan, M.B. and Bartek, J. (2004) Nature 432, 316–323. (7) Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39–85. (8) Guo, S. et al. (2006) J. Biol. Chem. Epub ahead of print. (9) Wu, X. et al. (2005) Oncogene 24, 4728–4735. (10) Binz, S.K. et al. DNA Repair (Amst) 3, 1015–1024. (11) Nuss, J.E. et al. (2005) Biochemistry 44, 8428–8437. (12) Yuzhakov, A. et al. (1999) EMBO J. 18, 6189–6199.

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