antibody

ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2, BDB, BDB1, MGC163394, Neurotrophic Tyrosine Kinase, Receptor-related 2, NTRKR2, Tyrosine-protein Kinase Transmembrane Receptor ROR2)

R2999-61

100 ul

polyclonal

domestic rabbit

nonconjugated

ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2, BDB, BDB1, MGC163394, Neurotrophic Tyrosine Kinase, Receptor-related 2, NTRKR2, Tyrosine-protein Kinase Transmembrane Receptor ROR2)

rabbit

Pab

Antibodies

100 ul

IgG

ROR1 and ROR2 are orphan receptor tyrosine kinases that are most closely related to MuSK and the Trk family of neurotrophin receptors. They are characterized by the presence of extracellular frizzled-like cysteine-rich domains and membrane-proximal kringle domains, both of which are assumed to mediate protein-protein interactions. The ROR family RTKs are evolutionarily conserved among Caenorhabditis elegans, Drosophila, mice, and humans. Although the functions of ROR kinases are unknown, similarities between ROR and MuSK and Trk kinases have led to speculation that ROR kinases regulate synaptic development. It was shown that CAM-1, a C. elegans ortholog of the ROR family RTKs, plays several important roles in regulating cellular migration, polarity of asymmetric cell divisions, and axonal outgrowth of neurons during nematode development. mROR1 and mROR2 may play differential roles during the development of the nervous system. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to residues close to the amino-terminal sequence of human ROR2.

Recognizes transfected levels of human ROR2 at ~135kD. Does not crossreact with ROR1. Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography Immunogen: Synthetic peptide corresponding to residues close to the amino-terminal sequence of human ROR2. Specificity: Recognizes transfected levels of human ROR2 at ~135kD. Does not crossreact with ROR1. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Al-Shawi, R., et al., Dev. Genes Evol. 211: 161–171 (2001). 2. Nomi, M., et al., Mol. Cell. Biol. 21: 8329–8335 (2001). 3. Roszmusz, E., et al., J. Biol. Chem. 276: 18,485–18,490 (2001). 4. Forrester, W.C., et al., Nature 400: 881–885 (1999). 5. Oishi, I., et al., Genes Cells 4: 41–56 (1999).

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Immunocytofluorescence staining of HEK293 cells (left) and HEK293 cells overexpressing mouse ROR2 (right), using R2999-61.