Rituximab | US Biological R2032-24B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Rituximab

catalog :

R2032-24B

quantity :

200 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

10F380 (MB2 A4)

product information

Catalog Number :

R2032-24B

Product wo Prefix :

Rituximab

Host :

rat

Product Type :

Mab

Category :

Antibodies

Size1 :

200 ug

Clone # USB :

10F380 (MB2 A4)

Isotype :

IgG2a

Desc1 :

Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. This antibody has been used to detect Rituximab bound to the surface of Raji B cell line. It is possible that complement disposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 50ug/ml; 10ul labels 10e6 cells in 100ul ELISA: 5ug/ml as coating antibody Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized rats were fused with cells of the NS-1 myeloma cell line. Recommended Negative Control: I1904-82D1: IgG2a, Rat Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

F(ab)2 fragment of Rituximab.

Specificity :

Recognizes the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. Does not recognize other CD20 antibodies.

Purity :

Purified by Protein G affinity chromatography from tissue culture supernatant.

Form :

Supplied as a liquid in TBS, pH 8.0, 0.09% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 10F380 (MB2 A4) Host: rat Concentration: ~1mg/ml Form: Supplied as a liquid in TBS, pH 8.0, 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography from tissue culture supernatant. Immunogen: F(ab)2 fragment of Rituximab. Specificity: Recognizes the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. Does not recognize other CD20 antibodies. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC

Storage Temperature :

-20°C

Reference :

1. Bayne, M. et al. (2003) Br. J. Cancer. 88:S38. 2. Cragg, M. S. et al. (2004) Blood. 103: 3989-3990. 3. Beum, P. V. et al. (2004) J. Immunol. Methods. 294: 37-42. 4. Hampson, G. et al. (2010) J. Immunol. Methods 360: 30-38. 5. Blasco, H. et al. (2007) J Immunol Methods. 325: 127-39. 6. Daydé, D. et al. (2009) Blood. 113: 3765-72. 7. Aung, T. et al. (2011) Proc Natl Acad Sci USA. 108: 15336-41. 8. Schmidt, E. et al. (2009) Clin Immunol. 132: 334-41. 9. McDonald, V. et al. (2010) J Thromb Haemost. 8: 1201-8. 10. Kagan, L. et al. (2011) Subcutaneous Absorption of Monoclonal Antibodies: Role of Dose, Site of Injection, and Injection Volume on Rituximab Pharmacokinetics in Rats. Pharm Res. Sep 2. [Epub ahead of print]