antibody

Rheb (Ras Homolog Enriched In Brain 2) discontinued

R1996-79A

100 ul

polyclonal

domestic rabbit

nonconjugated

Rheb (Ras Homolog Enriched In Brain 2) discontinued

rabbit

Pab

Antibodies

100 ul

IgG

Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberin's GAP activity (7). Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:400 Flow Cytometry: 1:50 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt Ye

Synthetic peptide corresponding to residues surrounding Asn50 of Rheb (KLH).

Recognizes endogenous levels of Rheb. Does not crossreact with other family members at physiological levels. Species Crossreactivity: human, mouse, rat and S. cerevisiae. Species sequence homology: bovine.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Asn50 of Rheb (KLH). Specificity: Recognizes endogenous levels of Rheb. Does not crossreact with other family members at physiological levels. Species Crossreactivity: human, mouse, rat and S. cerevisiae. Species sequence homology: bovine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IHC WB

-20°C

(1) Yamagata, K. et al. (1994) J. Biol. Chem. 269, 16333–16339. 2) Yee, W.M. and Worley, P.F. (1997) Mol. Cell. Biol. 17, 921–933. 3) Inoki, et al. (2003) Genes Dev. 17, 1829–1834. 4) Stocker, H. et al. (2003) Nature Cell Biol. 5, 559–565. 5) Saucedo, L.J. et al. (2003) Nat. Cell Biol. 5, 566–571. 6) Zhang, Y. et al. (2003) Nat. Cell Biol. 5, 578–581. 7) Li, Y. et al. (2004) Trends Biochem. Sci. 1, 32–38.

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