antibody

RBAP46/RBAP48 (Retinoblastoma Binding Protein 7, RbBP7, Rb46, RbAp46, Retinoblastoma Binding Protein p46, Retinoblastoma-associated Protein 46)

R1665-35B

100 ul

polyclonal

domestic rabbit

nonconjugated

RBAP46/RBAP48 (Retinoblastoma Binding Protein 7, RbBP7, Rb46, RbAp46, Retinoblastoma Binding Protein p46, Retinoblastoma-associated Protein 46)

rabbit

Pab

Antibodies

100 ul

IgG

Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAB46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and EZH2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to the N-terminus of the human RBAP48. Species sequence homology: chicken and equine (100%)

Recognizes endogenous levels of total RBAP46 and RBAP48 proteins Species Crossreactivity: mouse, monkey and rat

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the N-terminus of the human RBAP48. Species sequence homology: chicken and equine (100%) Specificity: Recognizes endogenous levels of total RBAP46 and RBAP48 proteins Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Qian, Y.W. et al. (1993) Nature 364, 648-52, 2. Verreault, A. et al. (1996) Cell 87, 95-104, 3. Verreault, A. et al. (1998) Curr Biol 8, 96-108, 4. Barak, O. et al. (2003) EMBO J 22, 6089-100, 5. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35, 6. Zhang, Y. et al. (1997) Cell 89, 357-64, 7. Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905, 8. Cao, R. et al. (2002) Science 298, 1039-43.

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