antibody

Retinoblastoma, phosphorylated (Ser807/811) (Rb)

R1660-05C

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Retinoblastoma, phosphorylated (Ser807/811) (Rb)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1- phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2–4). Cell cycle–dependent phosphorylation by cdk’s inhibits Rb target binding, thus allowing cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin Ecdk2 phosphorylation (6).Specificity of different cdk/cyclin complexes has been observed in vitro (6–8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:300 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Rt

Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser807/811 of human Rb.

Recognizes endogenous levels of human Rb only when phosphorylated at Ser807/811. Does not recognize Rb phosphorylated at other sites. Species Crossreactivity: Monkey and rat.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser807/811 of human Rb. Specificity: Recognizes endogenous levels of human Rb only when phosphorylated at Ser807/811. Does not recognize Rb phosphorylated at other sites. Species Crossreactivity: Monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC IP WB

-20°C

(1) Sherr, C.J. (1996) Science 274, 1672–1677. (2) Nevins, J. R. et al. (1992) Science 258, 424–429. (3) Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779–790. (4) Hu, Q. J. et al. (1990) EMBO J. 9, 1147–1155. (5) Knudsen, E.S. and Wang, J.Y. (1997) Mol. Cell. Biol. 17, 5771–5783. (6) Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753–761. (7) Connell-Crowley, L. et al. (1997) Mol. Cell. Biol. 8, 287–301. (8) Kitagawa, M. et al. (1996) EMBO J. 15, 7060–7069. (9) Geng, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 194–199. Burkitt lymphoma cells Rb-deficient cells Phospho-Rb (Ser807/811)

https://usbio-images.r.worldssl.net/prodimages/31/R1660-05C_1.jpg