antibody

Retinoblastoma, phosphorylated (Ser780) (Rb)

R1660-05A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Retinoblastoma, phosphorylated (Ser780) (Rb)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1- phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by cdk’s inhibits Rb target binding, thus allowing cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (6). Specificity of different cdk/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). Applications: Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Peptide ELISA: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Retinoblastoma.

Recognizes endogenous levels of human Retinoblastoma at ~110kD only when phosphorylated at Ser780. Does not recognize Rb phosphorylated at other sites. Species Crossreactivity: rat, monkey.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Retinoblastoma. Specificity: Recognizes endogenous levels of human Retinoblastoma at ~110kD only when phosphorylated at Ser780. Does not recognize Rb phosphorylated at other sites. Species Crossreactivity: rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

(1) Sherr, C.J. (1996) Science 274, 1672-1677. (2) Nevins, J.R. et al. (1992) Science 258, 424-429. (3) Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-790. (4) Hu, Q.J. et al. (1990) EMBO J. 9, 1147-1155. (5) Knudsen, E.S. and Wang, J.Y. (1997) Mol. Cell. Biol. 17, 5771-5783. (6) Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761. (7) Connell-Crowley, L. et al. (1997) Mol. Cell. Biol. 8, 287-301. (8) Kitagawa, M. et al. (1996) EMBO J. 15, 7060-7069. (9) Geng, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 194-199.

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