Ret, phosphorylated (Tyr905) | US Biological R1637 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Ret, phosphorylated (Tyr905)

catalog :

R1637

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

R1637

Product wo Prefix :

Ret, phosphorylated (Tyr905)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The Ret proto-oncogene (c-Ret), a receptor tyrosine kinase, functions as a multicompetent receptor complex in conjunction with other membrane bound ligand-binding GDNF family receptors (aGFRs) (1). The ligands for c-Ret have been identified as the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Gene alterations in c-Ret have been found to associate with different diseases including papillary thyroid carcinoma, multiple endocrine neoplasma type 2A (MEN2A), MEN2B, familial medullary thyroid carcinoma and a congenital developmental defect called Hirschsprungís disease (1,3). Tyr905 is located in the kinase domain of Ret and plays crucial roles in its catalytic and/or biological activities. Substitution of phenylalanine for Tyr905 dramatically inhibits its autophosphorylation activity (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Dr Hu

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret (KLH).

Specificity :

Recognizes endogenous levels of human Tyr905 phosphorylated Ret. Does not crossreact with other tyrosine-phosphorylated proteins. Species crossreactivity: Drosophila.

Purity :

Purified by Protein A and immunoaffinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret (KLH). Specificity: Recognizes endogenous levels of human Tyr905 phosphorylated Ret. Does not crossreact with other tyrosine-phosphorylated proteins. Species crossreactivity: Drosophila. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Airaksinen, M.S., et al., Mol. Cell. Neurosci. 13: 313–325 (1999). 2. Takahashi, M., et al., Oncogene 4: 805–806 (1989). 3. Manie, S., et al., Trends Genet. 17: 580–589 (2001). 4. Tallini, G. and Asa, S., Adv. Anat. Pathol. 8: 345–354 (2001). 5. Iwashita, T., et al., Oncogene 18: 3919–3922 (1999).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/21/R1637_1.jpg