RasGRP3 | US Biological R1198-10G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

RasGRP3

catalog :

R1198-10G

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C33A3

The same clone is also sold as:

Cell Signaling Technology: 3334

reactivity :

human

product information

Catalog Number :

R1198-10G

Product wo Prefix :

RasGRP3

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

C33A3

Isotype :

IgG

Desc1 :

Lymphocyte activation occurs in part through activation of the Ras signaling pathway following lymphocyte receptor stimulation. The RasGRP family of guanine nucleotide exchange factors (GEFs) catalyzes the exchange of GDP for GTP on Ras family small GTPases, promoting their active GTP-bound form. Diacylglycerol (DAG) or phorbol ester binding to RasGRP family members causes their translocation to the cell membrane and stimulates their activity (1,2). While T-Cells express RasGRP1, B-cells express both RasGRP1 and RasGRP3. RasGRP3 is important in linking B-cell receptor (BCR) activation to Ras signaling (3). In response to BCR stimulation, RasGRP3 is phosphorylated at Thr133 by PKC. This phosphorylation event further activates RasGRP3 in response to DAG, which stimulates PKC activity (4,5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to residues near the C-teminus of human RasGRP3. Species sequence homology: monkey (100%)

Specificity :

Recognizes endogenous levels of total human RasGRP3. Species Crossreactivity: mouse

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: C33A3 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the C-teminus of human RasGRP3. Species sequence homology: monkey (100%) Specificity: Recognizes endogenous levels of total human RasGRP3. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Braun, D.C. et al. (2005) Mol Cancer Ther 4, 141–50. (2) Lorenzo, P.S. et al. (2001) Cancer Res 61, 943–9. (3) Oh-hora, M. et al. (2003) J Exp Med 198, 1841–51. (4) Aiba, Y. et al. (2004) Proc Natl Acad Sci USA 101, 16612–7. (5) Zheng, Y. et al. (2005) Blood 105, 3648–54.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/21/R1198-10G_1.jpg