Ras, pan, N,H,K | US Biological R1198-01D product information

product summary

company name :

US Biological

product type :

antibody

product name :

Ras, pan, N,H,K

catalog :

R1198-01D

quantity :

100 ul

price :

516 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H56(27H5)

reactivity :

human

application :

western blot

more info or order :

US Biological product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5a	Friedrich T, Söhn M, Gutting T, Janssen K, Behrens H, Rocken C, et al. Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer. EBioMedicine. 2016;8:159-172 pubmed publisher

product information

Catalog Number :

R1198-01D

Product wo Prefix :

Ras, pan, N,H,K

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Size1 List :

516 USD

Clone # USB :

7H56(27H5)

Isotype :

IgG

Desc1 :

The 21kD guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Dr Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues near the amino terminus of human K-Ras.

Specificity :

Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity: mouse, monkey, rat and D. melanogaster

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H56(27H5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human K-Ras. Specificity: Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity: mouse, monkey, rat and D. melanogaster Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654. 2. Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. 3. Buday, L. and Downward, J. (1993) Cell 73, 611-620. 4. Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431. 5. Bos, J.L. (1989) Cancer Res. 49, 4682-4689. 6. Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/45/R1198-01D_1.jpg

more info or order :

US Biological product webpage