Ran (Androgen Receptor-associated Protein 24, ARA24, Gsp1, GTP Binding Nuclear Protein RAN, GTPase Ran, LPS, RAN Member RAS Oncogene Family, Ras-like Protein TC4, Ras-related Nuclear Protein, RASL2-8, TC4) | US Biological R1069-02 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Ran (Androgen Receptor-associated Protein 24, ARA24, Gsp1, GTP Binding Nuclear Protein RAN, GTPase Ran, LPS, RAN Member RAS Oncogene Family, Ras-like Protein TC4, Ras-related Nuclear Protein, RASL2-8, TC4)

catalog :

R1069-02

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

R1069-02

Product wo Prefix :

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport. During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation. In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with 1X TBS, 5% BSA. 0.1% Tween-20 at 4ºC with gentle shaking overnight. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hm Hu Mk Mo Rt

Immunogen :

Two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Species Sequence Homology: D. melanogaster and Xenopus, 100%

Specificity :

Recognizes endogenous levels of total human Ran protein. Does not crossreact with other small GTPases of the Ras family. Species Crossreactivity: mouse, rat, monkey, hamster and bovine

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Species Sequence Homology: D. melanogaster and Xenopus, 100% Specificity: Recognizes endogenous levels of total human Ran protein. Does not crossreact with other small GTPases of the Ras family. Species Crossreactivity: mouse, rat, monkey, hamster and bovine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Quimby, B.B. and Dasso, M. (2003) Curr. Opin. Cell Biol. 15, 338-344. 2. Hetzer, M. et al. (2002) Nat. Cell Biol. 4, 177-184. 3. Yamaguchi, R. and Newport, J. (2003) Cell 113, 115-125.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/R1069-02_1.jpg