antibody

Raf kinase inhibitor protein (Gly38) (RKIP)

R0495-55B

100 ul

polyclonal

domestic rabbit

nonconjugated

Raf kinase inhibitor protein (Gly38) (RKIP)

rabbit

Pab

Antibodies

100 ul

IgG

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-kB pathway, including NF-kB-inducing kinase (NIK), TAK1, IKKa and IKKB (5). These interactions antagonize cytokine-induced activation of the NF-kB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Gly38 of human RKIP protein.

Recognizes endogenous levels of total human RKIP. Species crossreactivity: mouse, rat, monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Gly38 of human RKIP protein. Specificity: Recognizes endogenous levels of total human RKIP. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Yeung, K. et al. (1999) Nature 401, 173-177, 2. Yeung, K. et al. (2000) Mol. Cell. Biol. 20, 3079-3085, 3. Corbit,, K. C. et al. (2003) J. Biol. Chem. 278,13061-13068, 4. Lorenz, K. et al. (2003) Nature 426, 574-579, 5. Yeung, K. C. et al. (2001) Mol. Cell. Biol. 21, 3079-3085, 6. Fu, Z. et al. (2003) J. Natl. Cancer Inst. 95, 878-889.

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