antibody

RAF1 (Raf-1, RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, cRaf, RAF)

R0495-06A

100 ug

polyclonal

domestic rabbit

nonconjugated

RAF1 (Raf-1, RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, cRaf, RAF)

rabbit

Pab

Antibodies

100 ug

IgG

Human Raf1 is a proto-oncogene-encoded 74kD kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-4ug/ml Immunoprecipitation: 15-25ug/ml Immunohistochemistry: 10-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to human Raf-1 at C-terminus.

Recognizes human Raf-1 at ~74kD. Species Crossreactivity: mouse and rat.

Purified by affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.

~0.1mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol. Purity: Purified by affinity chromatography. Immunogen: Synthetic peptide corresponding to human Raf-1 at C-terminus. Specificity: Recognizes human Raf-1 at ~74kD. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC IP WB

-20°C