antibody

Rad21 (Double Strand Break Repair Protein Rad21 Homolog, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear Matrix Protein 1, NXP-1, NXP1, RAD21 Homolog, SCC1, SCC1 Homolog)

R0447-02C

100 ul

polyclonal

domestic rabbit

nonconjugated

Rad21 (Double Strand Break Repair Protein Rad21 Homolog, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear Matrix Protein 1, NXP-1, NXP1, RAD21 Homolog, SCC1, SCC1 Homolog)

rabbit

Pab

Antibodies

100 ul

IgG

The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2 or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:25. Immunofluorescence (IF-IC): 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to human RAD21. Species Sequence Homology: porcine, chicken, bovine, equine (100%)

Recognizes endogenous levels of total human RAD21. Species crossreactivity: mouse, rat, monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to human RAD21. Species Sequence Homology: porcine, chicken, bovine, equine (100%) Specificity: Recognizes endogenous levels of total human RAD21. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IP WB

-20°C

1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114, 2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35, 3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67, 4. Hauf, S. et al. (2005) PLoS Biol 3, e69, 5. Hauf, S. et al. (2001) Science 293, 1320-3, 6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77, 7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.

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