Rac1/Cdc42 | US Biological R0400-05D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Rac1/Cdc42

catalog :

R0400-05D

quantity :

20 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

R0400-05D

Product wo Prefix :

Rac1/Cdc42

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

20 ul

Isotype :

IgG

Desc1 :

Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues near the N-termini of human rac1 and cdc42.

Specificity :

Recognizes endogenous levels of total rac1 and cdc42 protein. Based on sequence similarities, the antibody may react with other small GTPases. Species crossreactivity: Human, mouse, rat and monkey

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the N-termini of human rac1 and cdc42. Specificity: Recognizes endogenous levels of total rac1 and cdc42 protein. Based on sequence similarities, the antibody may react with other small GTPases. Species crossreactivity: Human, mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312. 2. Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85. 3. Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/8/R0400-05D_1.jpg