Rac1/cdc42, phosphorylated (Ser71) | US Biological R0400-05 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Rac1/cdc42, phosphorylated (Ser71)

catalog :

R0400-05

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

R0400-05

Product wo Prefix :

Rac1/cdc42, phosphorylated (Ser71)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Rac1 and cdc42 are members of the Rho-GTPase family. They play key roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. It has been established that GTP binding stimulates the activity of Rac1/cdc42. A putative Akt phosphorylation site at serine 71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (1). Phosphorylation at this site may inhibit GTP binding of Rac1, therefore attenuating the signal transduction pathway downstream of Rac1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42. Species Sequence Homology: mouse and rat, 100%

Specificity :

Recognizes endogenous levels of human Rac1/cdc42 when phosphorylated at Ser71. May also recognize phospho-RhoA (Ser73).

Purity :

Purified by Protein A and peptide affinity chromatography

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42. Species Sequence Homology: mouse and rat, 100% Specificity: Recognizes endogenous levels of human Rac1/cdc42 when phosphorylated at Ser71. May also recognize phospho-RhoA (Ser73). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Kwon, T. et al. (2000) J. Biol. Chem. 275, 423–428. 2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117: 1301-12. 3. Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14: 377-85. 4. Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6: 167-80.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/46/R0400-05_1.jpg