Rab8 discontinued | US Biological R0008-60D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Rab8 discontinued

catalog :

R0008-60D

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

R0008-60D

Product wo Prefix :

Rab8 discontinued

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring (1). Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane (1-3). Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation (4-6). Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle (7,8). Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB/MICAL-L2 at tight junction formation (9,10). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues surrounding Met173 of human Rab8 protein (KLH-coupled).

Specificity :

Recognizes human Rab8. Species crossreactivity: mouse, rat, monkey.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Host: rabbit Source: human Concentration: Not determined Form: Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Met173 of human Rab8 protein (KLH-coupled). Specificity: Recognizes human Rab8. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12, 2. Huber, L.A. et al. (1993) J Cell Biol 123, 35-45, 3. Henry, L. and Sheff, D.R. (2008) Mol Biol Cell 19, 2059-68, 4. Peränen, J. et al. (1996) J Cell Biol 135, 153-67, 5. Hattula, K. et al. (2002) Mol Biol Cell 13, 3268-80, 6. Hattula, K. et al. (2006) J Cell Sci 119, 4866-77, 7. Ishikura, S. and Klip, A. (2008) Am J Physiol Cell Physiol 295, C1016-25, 8. Randhawa, V.K. et al. (2008) J Biol Chem 283, 27208-19, 9. Bravo-Cordero, J.J. et al. (2007) EMBO J 26, 1499-510, 10. Yamamura, R. et al. (2008) Mol Biol Cell 19, 971-83.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/38/R0008-60D_1.jpg