Rab6 | US Biological R0008-03 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Rab6

catalog :

R0008-03

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

R0008-03

Product wo Prefix :

Rab6

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A’ and Rab6B) are structurally similar but likely exhibit nonoverlapping functions (2,3). Rab6 is localized to the Golgi (4) and regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6 related transport requires a functionally intact microtubule system (9,10). Rab6 proteins help regulate cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Lys133 of human Rab6. Uniprot Accession: P20340

Specificity :

Recognizes endogenous levels of total human Rab6. Species crossreactivity: mouse, rat, monkey.

Purity :

Purified by peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Lys133 of human Rab6. Uniprot Accession: P20340 Specificity: Recognizes endogenous levels of total human Rab6. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17, 2. Echard, A. et al. (2000) Mol Biol Cell 11, 3819-33, 3. Del Nery, E. et al. (2006) Traffic 7, 394-407, 4. Goud, B. et al. (1994) Ann N Y Acad Sci 733, 340-3, 5. White, J. et al. (1999) J Cell Biol 147, 743-60, 6. Opdam, F.J. et al. (2000) J Cell Sci 113 ( Pt 15), 2725-35, 7. Martinez, O. et al. (1997) Proc Natl Acad Sci USA 94, 1828-33, 8. Grigoriev, I. et al. (2007) Dev Cell 13, 305-14, 9. Echard, A. et al. (1998) Science 279, 580-5, 10. Young, J. et al. (2005) Mol Biol Cell 16, 162-77, 11. Hill, E. et al. (2000) EMBO J 19, 5711-9, 12. Miserey-Lenkei, S. et al. (2006) EMBO J 25, 278-89.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/8/R0008-03_1.jpg