antibody

PU.1 (Ets Transcription Factor)

P9190

100 ul

polyclonal

domestic rabbit

nonconjugated

PU.1 (Ets Transcription Factor)

rabbit

Pab

Antibodies

100 ul

IgG

PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box. PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes. The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation. In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by Casein Kinase II (CKII) promotes binding to IRF4 and synergistic activation through the immunoglobulin k 3' enhancer. Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1. GATA1 binding blocks PU.1 activity during erythroid cell development. Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Chromatin Immunoprecipitation: 1:25 Immunohistochemistry (Paraffin): 1:200. Antigen retrieval: Heat in citrate buffer, pH 6.0. Immunofluorescence (IF-IC): 1:100 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to amino acids at the N-terminus of human PU.1. Species Sequence Homology: monkey and porcine

Recognizes endogenous levels of total human PU.1 protein at ~42kD. Does not cross react with other Ets family members. Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to amino acids at the amino-terminus of human PU.1, conjugated to KLH. Species Sequence Homology: monkey and porcine Specificity: Recognizes endogenous levels of total human PU.1 protein at ~42kD. Does not cross react with other Ets family members. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

ChIP E FC IC IF IHC IP WB

-20°C

1. Lloberas, J. et al. (1999) Immunol. Today 20, 184–189. 2. Klemsz, M.J. et al. (1990) Cell 61, 113–124. 3. Dahl, R. and Simon, M.C. (2003) Blood Cells Mol. Dis. 31, 229–233. 4. DeKoter, R.P. and Singh, H. (2000) Science 288, 1439–1441. 5. Pongubala, J.M. et al. (1993) Science 259, 1622–1625. 6. Wang, J.M. et al. (2003) Mol. Cell. Biol. 23, 1896–1909. 7. Zhang, P. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8705–8710. 8. Moreau-Gachelin, F. et al. (1998) Nature 331, 277–280.

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Flow cytometric analysis of RAW cells, using P9190 (blue) compared to a nonspecific negative control antibody (red).

https://usbio-images.r.worldssl.net/prodimages/9/P9190_5.jpg

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (infiltrating cells), using P9190 in the presence of control peptide (left) or PU.1 Blocking Peptide (right).