antibody

PTEN Tumor Suppressor Protein, phosphorylated (Ser380/Thr382/383) (MMAC1, Phosphatase and Tensin, TEP1)

P9182-01E

20 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

7H54(44A7)

PTEN Tumor Suppressor Protein, phosphorylated (Ser380/Thr382/383) (MMAC1, Phosphatase and Tensin, TEP1)

rabbit

Mab

Phosphorylated

Antibodies

20 ul

7H54(44A7)

IgG

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also refered to as MMAC (mutated in multiple advanced cancers), phosphatase is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes the 403aa polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3 kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4–5). PTEN possesses a carboxy-terminal noncatalytic regulatory domain containing three phosphorylation sites (Ser380, Thr382 and Thr383), which regulates its stability and may play an important role in control of its biological activity (6,7). PTEN also regulates p53 protein levels and activity (8) and is involved in G protein coupled signaling during chemotaxis (9,10). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN. Species sequence homology: chicken (100%)

Recognizes endogenous levels of human PTEN only when phosphorylated at serine 380, threonine 382 and threonine 383. Species Crossreactivity: monkey, mouse and rat.

Purified

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 7H54(44A7) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN. Species sequence homology: chicken (100%) Specificity: Recognizes endogenous levels of human PTEN only when phosphorylated at serine 380, threonine 382 and threonine 383. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

(1) Cantley, L.C. and Neel, B.G. (1999) Proc. Natl. Acad. Sci. USA 96, 4240–4245. (2) Myers, M.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 9052–9057. (3) Myers, M.P. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 13513–13518. (4) Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205–8211. (5) Wu, X. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15587–15591 (6) Vazquez, F. et al. (2000) Mol. Cell. Biol. 20, 5010–5018. (7) Torres, J. and Pulido, R. (2001) J. Biol. Chem. 276, 993–998. (8) Freeman, D.J. et al. (2003) Cancer Cell 3, 117–130. (9) Funamoto, S. et al. (2002) Cell 109, 611–623. (10) Iijima, M. and Devreotes, P. (2002) Cell 109, 599–610.

https://usbio-images.r.worldssl.net/prodimages/34/P9182-01E_1.jpg