antibody

PTEN Tumor Suppressor Protein, phosphorylated (Ser380/Thr382/383) (MMAC1, Phosphatase and Tensin, TEP1)

P9182-01C

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

PTEN Tumor Suppressor Protein, phosphorylated (Ser380/Thr382/383) (MMAC1, Phosphatase and Tensin, TEP1)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

PTEN (phosphatase and tensin homologue deleted on chromosome ten)/MMAC (mutated in multiple advanced cancers) phosphatase is a tumor suppressor gene implicated in a wide variety of human cancers (1). PTEN encodes the 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3 kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4–5). PTEN possesses a C-terminal noncatalytic regulatory domain that contains three phosphorylation sites (Ser380, Thr382 and Thr383), which regulates its stability and could play an important role in control of its biological activity (6,7). PTEN also regulates p53 protein levels and activity (8) and is involved in G protein coupled signaling during chemotaxis (9,10). Application: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser380/Thr382/383 of human PTEN.

Recognizes endogenous levels of human PTEN phosphorylated at Ser380/Thr382/383. Species Crossreactivity: Mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser380/Thr382/383 of human PTEN. Specificity: Recognizes endogenous levels of human PTEN phosphorylated at Ser380/Thr382/383. Species Crossreactivity: Mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Cantley, L.C. & Neel, B.G. Proc. Natl. Acad. Sci. USA 96: 4240–4245 (1999). 2. Myers, M.P., et al., Proc. Natl. Acad. Sci. USA 94: 9052–9057 (1997). 3. Myers, M.P., et al., Proc. Natl. Acad. Sci. USA 95: 13,513–13,518 (1998). 4. Wan, X. & Helman, L.J., Oncogene 22: 8205-8211 (2003). 5. Wu, X., et al., Proc. Natl. Acad. Sci. USA 95: 15,587–15,591 (1998). 6. Vazquez, F., et al., Mol. Cell. Biol. 20: 5010–5018 (2000). 7. Torres, J. & Pulido, R., J. Biol. Chem. 276: 993–998 (2001). 8. Freeman, D.J., et al., Cancer Cell 3: 117-130 (2003). 9. Funamoto, S., et al., Cell 109: 611-623 (2002). 10. Iijima, M. & Devreotes, P., Cell 109: 599-610 (2002).

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