PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1) | US Biological P9182-01 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

catalog :

P9182-01

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P9182-01

Product wo Prefix :

PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

PTEN (phosphatase and tensin homologue deleted on chromosome ten)/MMAC (mutated in multiple advanced cancers) phosphatase is a tumor suppressor gene implicated in a wide variety of human cancers (1). PTEN encodes the 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3 kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4–6). PTEN possesses a C-terminal noncatalytic regulatory domain that contains three phosphorylation sites (Ser380, Thr382 and Thr383), which regulates its stability and could play an important role in control of its biological activity (7,8). Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Calc Crossreactivity :

Hm Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the C-terminus of human PTEN.

Specificity :

Detects endogenous levels of total 54kD PTEN protein. Does not crossreact with related proteins. Species Crossreactivity: human, mouse, rat, hamster, monkey.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminus of human PTEN. Specificity: Detects endogenous levels of total 54kD PTEN protein. Does not crossreact with related proteins. Species Crossreactivity: human, mouse, rat, hamster, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

(1) Cantley, L.C. and Neel, B.G. (1999) Proc. Natl. Acad. Sci. USA 96, 4240–4245. (2) Myers, M.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 9052–9057. (3) Myers, M.P. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 13513–13518. (4) Haas-Kogan, D. et al. (1998) Curr. Biol. 8, 1195–1198. (5) Stambolic, V. et al. (1998) Cell 95, 29–39. (6) Wu, X. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15587–15591. (7) Vazquez, F. et al. (2000) Mol. Cell. Biol. 20, 5010–5018. (8) Torres, J. and Pulido, R. (2001) J. Biol. Chem. 276, 993–998.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/13/P9182-01_1.jpg