Protein Tyrosine Phosphatase (PEST, PTP-PEST) | US Biological P9109-21 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Protein Tyrosine Phosphatase (PEST, PTP-PEST)

catalog :

P9109-21

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4i12(AG10)

reactivity :

human

product information

Catalog Number :

P9109-21

Product wo Prefix :

Protein Tyrosine Phosphatase (PEST, PTP-PEST)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

4i12(AG10)

Isotype :

IgG1

Desc1 :

PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASp, and other adhesion molecules (1–5). By modulating phosphorylation states of Shc, Pyk2, Fak and WASp, PTPPEST negatively regulates lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substates such as paxillin, p130 Cas, Crk and PSTPIP1 (8). Applications: Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Human PTP-PEST recombinant protein. Epitope: Within the amino-terminal 305 residues.

Specificity :

Recognizes endogenous levels of total human PTP-PEST protein at 110-125kD. Does not crossreact with other protein tyrosine phosphatases. Species Crossreactivity: mouse, rat and monkey.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 4i12(AG10) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Ascites Immunogen: Recombinant protein corresponding to human PTP-PEST. Epitope: Within the N-terminal 305 residues. Specificity: Recognizes endogenous levels of total human PTP-PEST protein at 110-125kD. Does not crossreact with other protein tyrosine phosphatases. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Davidson, D. and Veillette, A. (2001) EMBO J. 20, 3414–3426. 2. Garton, A.J. and Tonks, N.K. (1999) J. Biol. Chem. 274, 3811–3818. 3. Shen, Y. et al. (2000) J. Biol. Chem. 275, 1405–1413. 4. Angers-Loustau, A. et al. (1999) J. Cell Biol. 144, 1019–1031. 5. Cote, J. et al. (2002) J. Biol. Chem. 277, 2973–2986. 6. Badour, K. et al. (2003) J. Exp. Med. 199, 99–111. 7. Horsch, K. et al. (2001) Mol. Endocrinol. 15, 2182–2196. 8. Cong, F. et al. (2000) Mol. Cell 6, 1413–1423.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/P9109-21_1.jpg