antibody

Protein Synthesis Initiation Factor 4E (eIF 4E, Eukaryotic Translation Initiation Factor )

P9108-50D

100 ul

polyclonal

domestic rabbit

nonconjugated

Protein Synthesis Initiation Factor 4E (eIF 4E, Eukaryotic Translation Initiation Factor )

rabbit

Pab

Antibodies

100 ul

IgG

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure thereby mediating the initiation of translation (1,2). eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex (2). Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 has been shown to phosphorylate eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites have been identified in mouse Mnk1, Thr197 and Thr202, which are essential for Mnk1 kinase activity (3). The C-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). It is known that their phosphorylation is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. Applications: Suitable for use in ELISA, Western Blot and Immunohistchemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin and Frozen): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues around Ser209 of eIF4E (KLH).

Recognizes endogenous levels of total human eIF4E protein. The antibody does not cross-react with other proteins. Species Crossreactivity: mouse, rat, monkey and zebrafish.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide corresponding to residues around Ser209 of eIF4E (KLH). Specificity: Recognizes endogenous levels of total human eIF4E protein. The antibody does not cross-react with other proteins. Species Crossreactivity: mouse, rat, monkey and zebra fish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC WB

-20°C

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843–4847. 2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913–963. 3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871–1880. 4. Pyronnet, S. et al. (1999) EMBO J. 18, 270–279. 5. Raught, B. et al. (2000) EMBO J. 19, 434–444. 6. Banerjee, S. et al. (2002) Murine coronavirus replication- induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937–5948. 7. W. Krichevsky, A.M. and Kosik, K.S. (2001) Neuronal RNA granules: A link between RNA localization and stimulation- dependent translation. Neuron 32, 683–696.

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