antibody

Protein Phosphatase 2, Regulatory Subunit B, alpha (PPP2R2A , PP2A subunit B isoform alpha, PR55-alpha, Serine/threonine-protein phosphatase 2A 55kD regulatory subunit B alpha isoform)

P9107-44D

20 ul

monoclonal

domestic rabbit

nonconjugated

Protein Phosphatase 2, Regulatory Subunit B, alpha (PPP2R2A , PP2A subunit B isoform alpha, PR55-alpha, Serine/threonine-protein phosphatase 2A 55kD regulatory subunit B alpha isoform)

rabbit

Mab

Antibodies

20 ul

IgG

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by alpha and beta isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain alpha, beta, gamma and delta isoforms, with the B' family also including an epsilon protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:25 Immunohistochemistry (Paraffin): 1:100 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dr Hu Mk Mo Rt

Synthetic peptide derived from the sequence of human PP2A B subunit. Species sequence homology: chicken, bovine and porcine (100%)

Recognizes endogenous levels of the human PR55 PP2A B subunit (a isoform). May also recognize the b, g, and d isoforms of the PR55 PP2A B subunit. Does not cross-react with the B-prime (PR61), B-prime-prime or B-prime-prime-prime families of PP2A B subunits. Species Crossreactivity: mouse, rat, monkey, D. melanogaster

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide derived from the sequence of human PP2A B subunit. Species sequence homology: chicken, bovine and porcine (100%) Specificity: Recognizes endogenous levels of the human PR55 PP2A B subunit (a isoform). May also recognize the b, g, and d isoforms of the PR55 PP2A B subunit. Does not cross-react with the B-prime (PR61), B-prime-prime or B-prime-prime-prime families of PP2A B subunits. Species Crossreactivity: mouse, rat, monkey, D. melanogaster Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC IP WB

-20°C

1. Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39. 2. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35. 3. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91. 4. Chen, J. et al. (1992) Science 257, 1261-4. 5. Turowski, P. et al. (1995) J Cell Biol 129, 397-410. 6. Lee, J. et al. (1996) Proc Natl Acad Sci U S A 93, 6043-7. 7. Tolstykh, T. et al. (2000) EMBO J 19, 5682-91. 8. Yu, X.X. et al. (2001) Mol Biol Cell 12, 185-99.

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