Protein Kinase R (PKR, ds RNA-dependent Protein Kinase) | US Biological P9103-74 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Protein Kinase R (PKR, ds RNA-dependent Protein Kinase)

catalog :

P9103-74

quantity :

50 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P9103-74

Product wo Prefix :

Protein Kinase R (PKR, ds RNA-dependent Protein Kinase)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ul

Isotype :

IgG

Desc1 :

Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation. Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at Thr446 and Thr451 in the activation loop. Substitution of Thr451 to alanine completely inactivates PKR, while a mutant with a Thr446 to alanine substitution was partially active. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry (for Immunofluorescence). Other applications not tested. Recommended Dilution: Western Blot: 1:1,000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Immunocytochemistry (IC-IF): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues surrounding Thr451 of human PKR.

Specificity :

Recognizes endogenous levels of human PKR at 74kD. Does not crossreact with other related proteins.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Thr451 of human PKR. Specificity: Recognizes endogenous levels of human PKR at 74kD. Does not crossreact with other related proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC IF IP WB

Storage Temperature :

-20°C

Reference :

1. Williams, B.R. (1999) Oncogene 18, 6112-6120. 2. Gil, J. and Esteban, M. (2000) Apoptosis 5, 107-114. 3. Romano, P.R. et al. (1998) Mol. Cell. Biol. 18, 2282-2297.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/P9103-74_1.jpg