Protein Kinase C, theta, phosphorylated (Thr538) (PKCt) | US Biological P9103-68A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Protein Kinase C, theta, phosphorylated (Thr538) (PKCt)

catalog :

P9103-68A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

P9103-68A

Product wo Prefix :

Protein Kinase C, theta, phosphorylated (Thr538) (PKCt)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation and muscle contraction. PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG) and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG and phorbol esters. Members of these three PKC groups contain a pseudo- substrate or autoinhibitory domain that binds to substrate- binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation of Thr500 in the activation loop, the autophosphorylation site at Thr641 and at carboxy-terminal hydrophobic site Ser660 occurs in vivo. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Thr538 of human PKC theta. Species Sequence Homology: mouse; 100%

Specificity :

Recognizes endogenous levels of human PKC theta only when phosphorylated at Thr538. Species Crossreactivity: monkey and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr538 of human PKC theta. Species Sequence Homology: mouse; 100% Specificity: Recognizes endogenous levels of human PKC theta only when phosphorylated at Thr538. Species Crossreactivity: monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Nishizuka, Y. (1984) Nature 308, 693-698. 2. Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403. 3. Newton, A.C. (1995) J. Biol. Chem. 270, 28495-28498. 4. Mellor, H. and Perker, P.J. (1998) Biochem. J. 332, 281-292. 5. Ron, D. and Kazanietz, M.G. (1999) FASEB J. 13, 1658-1676. 6. Way, K.J. et al. (2000) Trends Pharmacol. Sci. 21, 181-187. 7. Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Reports 1, 399-403. 8. Good, J.A. (1998) Science 281, 2042-2045. 8. Good, J.A. et al. (1998) Science 281, 2042–2045. 9. Sonnenburg, E.D. (2001) J. Biol. Chem. 276, 45289–45297.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/P9103-68A_1.jpg

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/P9103-68A_6.jpg

Picture6 Text :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing membrane and cytoplasmic localization, using P9103-68A.