Protein Kinase C, epsilon (PKCe) | US Biological P9103-20Z product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Protein Kinase C, epsilon (PKCe)

catalog :

P9103-20Z

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8A28(22B10)

reactivity :

human

product information

Catalog Number :

P9103-20Z

Product wo Prefix :

Protein Kinase C, epsilon (PKCe)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8A28(22B10)

Isotype :

IgG

Desc1 :

Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to human PKCe. Species Sequence Homology: chicken (100%)

Specificity :

Recognizes endogenous levels of total human PKCe protein. Does not cross-react with other PKC isoforms. Species Crossreactivity: monkey, mouse and rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A28(22B10) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to human PKCe. Species Sequence Homology: chicken (100%) Specificity: Recognizes endogenous levels of total human PKCe protein. Does not cross-react with other PKC isoforms. Species Crossreactivity: monkey, mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Nishizuka, Y. (1984) Nature 308, 693-8. 2. Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403. 3. Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92. 4. Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76. 5. Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403. 6. Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8. 7. Flynn, P. et al. (2000) J Biol Chem 275, 11064-70.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/P9103-20Z_1.jpg