This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
protein A
catalog :
P9102-15A
quantity :
1 mg
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
product information
Catalog Number :
P9102-15A
Product wo Prefix :
protein A
Host :
chicken
Product Type :
Pab
Category :
Antibodies
Size1 :
1 mg
Isotype :
IgY
Desc1 :
Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each Protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
Protein A may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. These conjugates may be used to track immunoglobulins.
Alternatively, Protein A may be immobilized onto a solid support to facilitate the purification and recovery of either polyclonal or monoclonal immunoglobulins. Immobilized Protein A has recently been applied therapeutically to treat a variety of humoral diseases (Watson et al., 1989).
Applications:
Suitable for use in ELISA, Western Blot and Immunodiffusion. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Specificity :
Recognizes Protein A. One precipitin arc in Immunodiffusion against Staphylococcal Protein A.
Purity :
Purified by affinity chromatography.
Form :
Supplied as a liquid PBS, pH 7.4, 0.02% sodium azide.
Concentration :
~1mg/ml
Desc2 :
Product Type: Pab
Isotype: IgY
Host: chicken
Concentration: ~1mg/ml
Form: Supplied as a liquid PBS, pH 7.4, 0.02% sodium azide.
Purity: Purified by affinity chromatography.
Specificity: Recognizes Protein A. One precipitin arc in Immunodiffusion against Staphylococcal Protein A.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E WB
Storage Temperature :
4°C Do not freeze
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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