antibody

Proteasome alpha 2 (PSMA2)

P9076-03

100 ul

polyclonal

domestic rabbit

nonconjugated

Proteasome alpha 2 (PSMA2)

rabbit

Pab

Antibodies

100 ul

IgG

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with 7 distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5).PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome which preferentially degrades poly-ubiquitinylated proteins (1-3). The proteasome has a broad substrate spectrum which includes cell cycle regulators, signaling molecules, tumor suppressors and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune response, protein folding and disease progression (6-9). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Thr223 of human PSMA2 protein.

Detects endogenous levels of total human PSMA2 protein. Species Crossreactivity: Monkey, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Thr223 of human PSMA2 protein. Specificity: Detects endogenous levels of total human PSMA2 protein. Species Crossreactivity: Monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF WB

-20°C

1 Dahlmann, B. (2005) Essays Biochem. 41, 31-48. 2 Pickart, C.M. and Cohen, R.E. (2004) Nat. Rev. Mol. Cell Biol. 5, 177-187. 3 Nandi, D. et al. (2006) J. Biosci. 31, 137-155. 4 Lupas, A. et al. (1993) Enzyme Protein 47, 252-273. 5 Monaco, J.J. and Nandi, D. (1995) Annu. Rev. Genet. 29, 729-754. 6 Murray, A.W. (2004) Cell 116, 221-234. 7 Ciechanover, A. (2006) Proc. Am. Thorac. Soc. 3, 21-31. 8 Wang, J. and Maldonado, M.A. (2006) Cell. Mol. Immunol. 3, 255-261. 9 Rubinsztein, D.C. (2006) Nature 443, 780-786.

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