antibody

PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9, Neural Apoptosis-regulated Convertase 1, NARC-1, Proprotein Convertase 9, PC9, Subtilisin/Kexin-like Protease PC9, NARC1, PSEC0052)

P9052-40F

100 ug

polyclonal

domestic goat

nonconjugated

PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9, Neural Apoptosis-regulated Convertase 1, NARC-1, Proprotein Convertase 9, PC9, Subtilisin/Kexin-like Protease PC9, NARC1, PSEC0052)

goat

Pab

Antibodies

100 ug

IgG

PCSK9 is a serine protease of the protein convertase family having profound effects on plasma low-density lipoprotein (LDL)-cholesterol (LDL-C) levels through its ability to mediate LDL receptor (LDLR) protein degradation. The protein slows down the uptake of cholesterol by degrading LDLR after they have internalized LDL and thus plays a significant role in cholesterol homeostasis. PCSK9 also potentially averts excessive cholesterol accumulation within the cell by preventing the recycling of LDLRs to the cell surface. Structurally, the protein includes a signal peptide, followed by a prodomain, a subtilisin-like catalytic domain, and a variable C-terminal domain. The prodomain serves a dual role as a chaperone for folding and as an inhibitor of catalytic activity. Human PCSK9 gene is localized in the chromosomal region 1p32.3. Several mutations (p.S127R, p.F216L, p.D374Y) of this gene have been reported to cause hypercholesterolemia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.1-0.5ug/ml, transfected HEK293 transiently expressing full-length Human PCSK9 (myc and DYKDDDDK tagged) observed on ~80-100kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to TRFHRQASKCDSHGT, from human PCSK9, at the internal region of the protein (NP_777596.2).

Recognizes human PCSK9. Species sequence homology: mouse and rat.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to TRFHRQASKCDSHGT, from human PCSK9, at the internal region of the protein (NP_777596.2). Specificity: Recognizes human PCSK9. Species sequence homology: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Kwon HJ, Lagace TA, McNutt MC, Horton JD, Deisenhofer J, Molecular basis for LDL receptor recognition by PCSK9. Proc. Natl. Acad. Sci. U.S.A. 2008 Feb 105(6):1820-5.

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