antibody

PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9, Neural Apoptosis-regulated Convertase 1, NARC-1, Proprotein Convertase 9, PC9, Subtilisin/Kexin-like Protease PC9, NARC1, PSEC0052)

P9052-40E

100 ug

polyclonal

domestic goat

nonconjugated

PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9, Neural Apoptosis-regulated Convertase 1, NARC-1, Proprotein Convertase 9, PC9, Subtilisin/Kexin-like Protease PC9, NARC1, PSEC0052)

goat

Pab

Antibodies

100 ug

IgG

PCSK9 is a serine protease of the protein convertase family having profound effects on plasma low-density lipoprotein (LDL)-cholesterol (LDL-C) levels through its ability to mediate LDL receptor (LDLR) protein degradation. The protein slows down the uptake of cholesterol by degrading LDLR after they have internalized LDL and thus plays a significant role in cholesterol homeostasis. PCSK9 also potentially averts excessive cholesterol accumulation within the cell by preventing the recycling of LDLRs to the cell surface. Structurally, the protein includes a signal peptide, followed by a prodomain, a subtilisin-like catalytic domain, and a variable C-terminal domain. The prodomain serves a dual role as a chaperone for folding and as an inhibitor of catalytic activity. Human PCSK9 gene is localized in the chromosomal region 1p32.3. Several mutations (p.S127R, p.F216L, p.D374Y) of this gene have been reported to cause hypercholesterolemia. Applications: Suitable for use in Peptide ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000 Western Blot: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to aa164-175, C-PRYRADEYQPPD, at the internal region of the protein from human PCSK9 (NP_777596.2).

Recognizes human PCSK9.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide Purity: Purified by immunoaffinity chromatography Immunogen: Synthetic peptide corresponding to aa164-175, C-PRYRADEYQPPD, at the internal region of the protein from human PCSK9 (NP_777596.2). Specificity: Recognizes human PCSK9. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Lalanne F, Lambert G, Amar MJ, Chetiveaux M, Zair Y, Jarnoux AL, Ouguerram K, Friburg J, Seidah NG, Brewer HB Jr, Krempf M, Costet P. Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells. J Lipid Res. 2005 Jun;46(6):1312-9. Epub 2005 Mar 1.

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