Prolactin Receptor (PRLR, PRL-R, PRL Receptor) | US Biological P9009-56B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Prolactin Receptor (PRLR, PRL-R, PRL Receptor)

catalog :

P9009-56B

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P9009-56B

Product wo Prefix :

Prolactin Receptor (PRLR, PRL-R, PRL Receptor)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Prolactin (PRL) is a peptide hormone that exhibits high homology to growth hormone and is related to the peptide hormones of the interleukin family. The endocrine effects of PRL on human breast tissues include the regulation of growth and differentiation of ductal epithelium, proliferation and differentiation of lobular units, and initiation and maintenance of lactation. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of prolactin receptor (PRLr), a member of the cytokine receptor family. Heterogeneous expression of PRLr at the immunoblot and immuno- histochemical levels was observed in breast carcinoma specimens, with a statistically significant correlation between prolactin receptor and estrogen receptor expression. The quantitation of growth factor receptors such as estrogen and progesterone has been useful in the prognosis of human breast cancer. The presence of these receptors has also provided the basis for endocrine-based therapies, including tamoxifen. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit is ~2ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 5-15ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

NS0-derived recombinant protein corresponding to human Prolactin R, Gln25-Asp234, at the extracellular domain

Specificity :

Recognizes human Prolactin Receptor. Displays ~25% crossreactivity with recombinant mouse Prolactin Receptor and recombinant rat Prolactin Receptor in Western Blot.

Purity :

Purified by affinity chromatography. Endotoxin: ≤0.1EU/ug.

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

Concentration :

~0.1mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.1mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS. Purity: Purified by affinity chromatography. Endotoxin: ≤0.1EU/ug. Immunogen: NS0-derived recombinant protein corresponding to human Prolactin R, Gln25-Asp234, at the extracellular domain Specificity: Recognizes human Prolactin Receptor. Displays ~25% crossreactivity with recombinant mouse Prolactin Receptor and recombinant rat Prolactin Receptor in Western Blot. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C