antibody

Progesterone Receptor, phosphorylated (Ser190)

P9007-15F

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Progesterone Receptor, phosphorylated (Ser190)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B (1,2). Both PR-A and PR-B are ligand activated but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation, and at least seven serine residues are phosphorylated in its N-terminal domain. Three sites (serine 81, 102 and 162) are unique to full length PR-B and others (serine 190, 294, 345 and 400) are shared by both isoforms (5). The phosphorylation of Ser190 was demonstrated to be catalyzed by cdk2 in PR-B (equivalent to Ser26 of PR-A) (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation of Ser190 may be critical to its biological function. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Ser190 of human progesterone receptor (KLH).

Detects endogenous levels of human progesterone receptor B and A forms only when phosphorylated at Ser190 and Ser26, respectively. Does not crossreact with other PR family members.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser190 of human progesterone receptor (KLH). Specificity: Detects endogenous levels of human progesterone receptor B and A forms only when phosphorylated at Ser190 and Ser26, respectively. Does not crossreact with other PR family members. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

(1) Evans, R. M. et al. (1998) Science 240, 889–895. (2) Kastner, P. et al. (1990) EMBO J. 112, 1603–1614. (3) Giangrande, P. H. et al. (2000) Mol. Cell. Biol. 20, 3102–3115. (4) Wen, D. X. et al. (1994) Mol. Cell. Biol. 14, 8356–8364. (5) Clemm, D. L. et al. (2000) Mol. Endocrinol. 14, 52–65. (6) Zhang, X. et al. (1997) Mol. Endocrinol. 11, 823–832. (7) Takamoto, G. S. et al. (1996) J. Biol. Chem. 271, 13308–13316.

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