OCT-1 (POU2F1, POU Class 2 Homeobox 1, NF-A1, Octamer-binding Transcription Factor 1, OCT1, POU Domain, Class 2, Transcription Factor 1 OTF1, OTF-1) | US Biological P5250-01E product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

OCT-1 (POU2F1, POU Class 2 Homeobox 1, NF-A1, Octamer-binding Transcription Factor 1, OCT1, POU Domain, Class 2, Transcription Factor 1 OTF1, OTF-1)

catalog :

P5250-01E

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

P5250-01E

Product wo Prefix :

OCT-1 (POU2F1, POU Class 2 Homeobox 1, NF-A1, Octamer-binding Transcription Factor 1, OCT1, POU Domain, Class 2, Transcription Factor 1 OTF1, OTF-1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA- binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to amino acids surrounding Leu362 of human Oct-1. Uniprot Accession: P14859

Specificity :

Recognizes endogenous levels of human total Oct-1.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to amino acids surrounding Leu362 of human Oct-1. Uniprot Accession: P14859 Specificity: Recognizes endogenous levels of human total Oct-1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Sturm, R.A. et al. (1988) Genes Dev 2, 1582-99, 2. Mittal, V. et al. (1996) Mol Cell Biol 16, 1955-65, 3. Strubin, M. et al. (1995) Cell 80, 497-506, 4. Nakshatri, H. et al. (1995) J Biol Chem 270, 19613-23, 5. Zwilling, S. et al. (1994) Nucleic Acids Res 22, 1655-62, 6. Schonemann, M.D. et al. (1998) Adv Exp Med Biol 449, 39-53.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/7/P5250-01E_1.jpg