Polymyxin B | US Biological P4400-15C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Polymyxin B

catalog :

P4400-15C

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9G252 (45)

product information

Catalog Number :

P4400-15C

Product wo Prefix :

Polymyxin B

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

1 ml

Clone # USB :

9G252 (45)

Isotype :

IgM

Desc1 :

The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody enables the possibilities to study quantitatively the interaction of PMB and LPS. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:10 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Alternate Name :

PMB

Immunogen :

Whole bacteria of the Re chemotype of S. minnesota

Specificity :

Recognizes Polymyxin B. Binds to free Polymyxin B as well as to Polymyxin B already bound to LPS.

Purity :

Supernatant

Form :

Supplied as a liquid, 0.02% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgM Clone No: 9G252 (45) Host: mouse Concentration: Not determined. Form: Supplied as a liquid, 0.02% sodium azide. Purity: Supernatant Immunogen: Whole bacteria of the Re chemotype of S. minnesota Specificity: Recognizes Polymyxin B. Binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Appelmelk, B et al; Polymyxin B-horseradish pero1992, 207: 311