antibody

Plastin L (PLSL, L Plastin, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2)

P4268-01B

100 ul

polyclonal

domestic rabbit

nonconjugated

Plastin L (PLSL, L Plastin, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2)

rabbit

Pab

Antibodies

100 ul

IgG

Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mam-mals and include L-plastin, T-plastin and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues while I- plastin (plastin-1) is expressed specifically in kidney, colon and small intestine (1-3). L-plastin (plastin-2) or LCP1 (lymphocyte cytosolic protein 1) is mainly expressed in hematopoietic cells and non-hematopoietic tumors, including increased expression correlated with metastatic progression in colon cancer cell lines (4). Overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T-cell receptor/co-receptor complex (TCR/CD3) in association with the CD2 cell adhe-sion molecule or the CD28 receptor (6). Phosphorylation at Ser5 increases LCP1 ability to bind F-actin and increases cell motility (7,8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to residues surrounding Asp292 of human LCP1. Species sequence homology: rat (100%). Uniprot Accession: P13796

Recongnizes endogenous levels of human total LCP1 protein. Species Crossreactivity: Mouse.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Asp292 of human LCP1. Species sequence homology: rat (100%). Uniprot Accession: P13796 Specificity: Recongnizes endogenous levels of human total LCP1 protein. Species Crossreactivity: Mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92, 2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67, 3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79, 4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81, 5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104, 6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62, 7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60, 8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.

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