antibody

Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (PE)

P4258-23B

100Tests

monoclonal

mouse

PE

7H36

Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (PE)

mouse

Mab

PE

Antibodies

100Tests

7H36

IgG1

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts, and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimer are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Two distinct PDGF receptors, the a-receptor and the b-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the b-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using MG-63 human osteosarcoma line. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Hu Mk

Human skin fibroblast membrane extracts.

Recognizes human Platelet Derived Growth Factor Receptor, Type B. Species Crossreactivity: monkey, baboon. No reactivity with rat or mouse receptors is observed. Does not recognize the PDGF Ralpha subunit.

Purified by Protein G affinity chromatography from ascites.

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (PE).

~0.025mg/ml

Product Type: Mab Isotype: IgG1 Clone No: 7H36 Host: mouse Source: human Concentration: ~0.025mg/ml Form: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (PE). Purity: Purified by Protein G affinity chromatography from ascites. Immunogen: Human skin fibroblast membrane extracts. Specificity: Recognizes human Platelet Derived Growth Factor Receptor, Type B. Species Crossreactivity: monkey, baboon. No reactivity with rat or mouse receptors is observed. Does not recognize the PDGF Ralpha subunit. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC

4°C Do not freeze

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