PDGF Receptor Alpha | US Biological P4258-21Z product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDGF Receptor Alpha

catalog :

P4258-21Z

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8A27(D1E1E)

reactivity :

human

product information

Catalog Number :

P4258-21Z

Product wo Prefix :

PDGF Receptor Alpha

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8A27(D1E1E)

Isotype :

IgG

Desc1 :

The proteins of the platelet derived growth factor (PDGF) family consist of several disulphide-bonded dimeric isoforms: PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD, which bind in a distinct pattern to two highly related RTKs: PDGF receptor a (PDGFRa) and and PDGF receptor b (PDGFRb). PDGFRa and PDGFRb contain between 75% and 85% sequence homology between their two intracellular kinase domains. PDGFRb binds all PDGF isoforms except PDGF D, whereas PDGFRb binds only PDGF B and D. PDGFRa and PDGFRb not only form homo and heterodimers, but also dimerize with EGFR, which can be stimulated by PDGF. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules including Grb2, Src, GAP, PI3 kinase, PLCg and Nck. A number of different signaling pathways are initiated leading to cell growth, actin reorganization, migration and differentiation. Tyr751 in the kinase-insert region of PDGFRb is the docking site for PI3 kinase. Phosphorylated pentapeptides derived from Tyr751 of PDGFRb (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRb. Tyr740 is also required for PDGFRb mediated PI3 kinase activation. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:1000. Antigen retrieval: Heat in citrate buffer, pH 6.0. Immunoprecipitation: 1:50 Immunofluorescence (IC): 1:1000 Flow Cytometry: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to residues near the C- terminus of human PDGFRa.

Specificity :

Recognizes endogenous levels of human PDGFRa at ~190kD. This antibody may cross-react with PDGFRb at overexpressed levels. Species Crossreactivity: mouse

Purity :

Serum

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A27(D1E1E) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Serum Immunogen: Synthetic peptide corresponding to residues near the carboxy- terminus of human PDGFRa. Specificity: Recognizes endogenous levels of human PDGFRa at ~190kD. This antibody may cross-react with PDGFRb at overexpressed levels. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Deuel, T.F. et al. (1988) Biofactors 1, 213–217. 2. Betsholtz, C. et al. (2001) Bioessays 23, 494–507. 3. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 9–45. 4. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1–38. 5. Panayotou, G. et al. (1992) EMBO J. 11, 4261–4272. 6. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263–1272. 7. Kashishian, A. et al. (1992) EMBO J. 11, 1373–1382.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/48/P4258-21Z_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded U-118MG xenograft using P4258-21Z in the presence of control peptide (left) or antigen specific peptide (right).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/48/P4258-21Z_5.jpg

Picture5 Text :

Immunohistochemical analysis of paraffin-embedded HCC827 xenograft using P4258-21Z