PDGF Receptor alpha/beta, phosphorylated (Tyr849/Tyr857) | US Biological P4258-21Y product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

PDGF Receptor alpha/beta, phosphorylated (Tyr849/Tyr857)

catalog :

P4258-21Y

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

8A26(C43E9)

reactivity :

human

product information

Catalog Number :

P4258-21Y

Product wo Prefix :

PDGF Receptor alpha/beta, phosphorylated (Tyr849/Tyr857)

Host :

rabbit

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8A26(C43E9)

Isotype :

IgG

Desc1 :

The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFRa) and PDGF receptor beta (PDGFRb). PDGFRa and PDGFRb share 75%-85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27%-28%) of homology. PDGFRa homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRb homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGFa/b receptor binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer. PDGFRa and PDGFRb can each form heterodimers with EGFR, which is also activated by PDGF. Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding. Ligand binding induces receptor dimerization and auto-phosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration and differentiation. Tyr751 in the kinase-insert region of PDGFRb is the docking site for PI3 kinase. Phosphorylated pentapeptides derived from Tyr751 of PDGFRb inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRb. Tyr740 is also required for PDGFRb mediated PI3 kinase activation. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Tyr857 of human PDGF receptor beta.

Specificity :

Recognizes endogenous levels of human PDGF receptor alpha and beta at ~190kD only when phosphorylated on Tyr849 of PDGFR alpha and Tyr857 of PDGFR beta. May crossreact with other activated tyrosine kinases. Species Crossreactivity: Mouse, rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A26(C43E9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr857 of human PDGF receptor beta. Specificity: Recognizes endogenous levels of human PDGF receptor alpha and beta at ~190kD only when phosphorylated on Tyr849 of PDGFR alpha and Tyr857 of PDGFR beta. May crossreact with other activated tyrosine kinases. Species Crossreactivity: Mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Deuel, T.F. et al. (1988) Biofactors 1, 213-217. 2. Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516. 3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507. 4. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45. 5. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38. 6. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272. 7. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272. 8. Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/P4258-21Y_1.jpg