antibody

Platelet Derived Growth Factor Receptor beta, phosphorylated (Tyr751) (PDGFRb)

P4258-02A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Platelet Derived Growth Factor Receptor beta, phosphorylated (Tyr751) (PDGFRb)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Platelet-derived growth factor (PDGF) exerts its stimulatory effects on cell growth and motility by binding to two related protein tyrosine kinase receptors, which can associate as three dimer forms (alpha/ alpha, alpha/beta, beta/beta) with variable binding specificity for the PDGF ligands (1,2). The total number of the two receptor subunits and the ratio expressed varies between cell types and appears to account for the variable responsiveness of different cell types to PDGF (3). Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain–containing signal transduction molecules including Grb2, Src, GAP, PI3 kinase, PLCgamma and Nck. A number of different signaling pathways are thereby initiated leading to cell growth, actin reorganization, migration and differentiation (4). Tyr751 in the kinase-insert region of PDGFR beta is the docking site for PI3 kinase (5). Phosphorylated pentapeptides derived from tyrosine 751 of the PDGFR beta (pTyr751-Val-Pro-Met-Leu) inhibit the association of the C-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFR beta (6). Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Flow Cytometry: 1:200 Optimal dilution to be determined by researcher Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr751of human PDGF receptor beta.

Detects endogenous levels of PDGF receptor beta only when phosphorylated at Tyr751 (190kD). Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Human, mouse, rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr751of human PDGF receptor beta. Specificity: Detects endogenous levels of PDGF receptor beta only when phosphorylated at Tyr751 (190kD). Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Human, mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC WB

-20°C

1. Deuel, T.F. et al. (1988) Biofactors 1, 213-217. 2. Betsholtz, C. et al. (2001) Bioessays 23, 494-507. 3. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45. 4. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38. 5. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272. 6. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272.

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