Phosphotyrosine | US Biological P4079G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine

catalog :

P4079G

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9F636

product information

Catalog Number :

P4079G

Product wo Prefix :

Phosphotyrosine

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

9F636

Isotype :

IgG2b

Desc1 :

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:4000 Western Blot: 1:4000 Immunohistochemistry: 1:4000 Immunoprecipitation: 1:4000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Hapten conjugated to phosphotyrosine

Specificity :

Recognizes tyrosine-phosphorylated protein in an EGF-stimulated A431 cell lysate. Does not crossreact with tyrosine, phosphoserine, or phosphothreonine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 9F636 Host: mouse Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.2. Purity: Purified by Protein G affinity chromatography. Immunogen: Hapten conjugated to phosphotyrosine Specificity: Recognizes tyrosine-phosphorylated protein in an EGF-stimulated A431 cell lysate. Does not crossreact with tyrosine, phosphoserine, or phosphothreonine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Reyes, L. et al (2002) J. Immunol. 169,5881- 5888.