Phosphotyrosine (HRP) | US Biological P4079D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (HRP)

catalog :

P4079D

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

5K212

product information

Catalog Number :

P4079D

Product wo Prefix :

Phosphotyrosine (HRP)

Host :

mouse

Product Type :

Mab

Conjugate :

HRP

Category :

Antibodies

Size1 :

1 ml

Clone # USB :

5K212

Isotype :

IgG2b,k

Desc1 :

Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. Also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of this clone for phosphotyrosine is approximately 10e-6 to 10e-7 M. Binding of this Ab to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.1-3 Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:4000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Phosphotyrosine (HRP).

Specificity :

Recognizes phosphorylated tyrosine and phenylphosphate.

Purity :

Purified

Form :

Supplied as a liquid in 50% PBS, pH 7.4, 50% glycerol. Labeled with HRP. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG2b,k Clone No: 5K212 Host: mouse Concentration: Not determined. Form: Supplied as a liquid in 50% PBS, pH 7.4, 50% glycerol. Labeled with HRP. No preservative added. Purity: Purified Immunogen: Phosphotyrosine (HRP). Specificity: Recognizes phosphorylated tyrosine and phenylphosphate. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF WB

Storage Temperature :

4°C (-20°C Glycerol)

Reference :

1. Glenney, J. R., Jr., L. Zokas, and M. P. Kamps. 1988. J. Immunol. Methods 109:277. 2. Ruff-Jamison, S., R. Campos-Gonzalez, and J. R. Glenney, Jr. 1991. J. Biol. Chem. 266:6607. 3. Glenney, J. R., Jr., W. S. Chen, C. S. Lazar, G. M. Walton, L. M. Zokas, M. G. Rosenfeld, and G. N. Gill. 1988. Cell 52:675. 4. Takagi, S., M. Daibata, T. J. Last, R. E. Humphreys, D. C. Parker, and T. Sairenji. 1991. J. Exp. Med 174:381.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/41/P4079D_1.jpg