Phosphotyrosine (AP) | US Biological P4079C product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Phosphotyrosine (AP)

catalog :

P4079C

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

PY20

product information

Catalog Number :

P4079C

Product wo Prefix :

Phosphotyrosine (AP)

Host :

mouse

Product Type :

Mab

Conjugate :

Category :

Antibodies

Size1 :

1 ml

Clone # USB :

PY20

Isotype :

IgG2b,k

Desc1 :

Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. Also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of this clone for phosphotyrosine is approximately 10e-6 to 10e-7 M. Binding of this Ab to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.1-3 Applications: • Detection of phosporylated tyrosine-containing proteins on Western blots 1 • Immunoaffinity purification of tyrosine-phosporylated proteins 1 • Quantitation of phosphotyrosine by Enzyme-Linked-Immunosorbent-Assay (ELISA)1 • Immunofluorescence analysis 4 Recommended Dilution: ELISA: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Specificity :

Recognizes phosphorylated tyrosine and phenylphosphate.

Purity :

Purified

Form :

Supplied as a liquid in 50mM Tris, 1mM MgCl2, 50% Glycerol, pH 8.0, containing 0.09% sodium azide.

Concentration :

As reported

Desc2 :

Product Type: Mab Isotype: IgG2b,k Clone No: PY20 Host: mouse Concentration: As reported Form: Supplied as a liquid in 50mM Tris, 1mM MgCl2, 50% Glycerol, pH 8.0, containing 0.09% sodium azide. Purity: Purified Specificity: Recognizes phosphorylated tyrosine and phenylphosphate. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF WB

Storage Temperature :

4°C Do Not Freeze

Reference :

1. Glenney, J. R., Jr., L. Zokas, and M. P. Kamps. 1988. J. Immunol. Methods 109:277. 2. Ruff-Jamison, S., R. Campos-Gonzalez, and J. R. Glenney, Jr. 1991. J. Biol. Chem. 266:6607. 3. Glenney, J. R., Jr., W. S. Chen, C. S. Lazar, G. M. Walton, L. M. Zokas, M. G. Rosenfeld, and G. N. Gill. 1988. Cell 52:675. 4. Takagi, S., M. Daibata, T. J. Last, R. E. Humphreys, D. C. Parker, and T. Sairenji. 1991. J. Exp. Med 174:381.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/P4079C_1.jpg